A Profiling for Forensic and Kinship Cases Studies.
The GT InDel Detector is based on Insertion/Deletion (ID) polymorphism phenomenon an alternative approach to STR based human identification. Insertion/Deletion (InDel) mutations are common in the human genome. InDels can be from a single base pair to several hundred bases or even more. Sometimes these InDels are stable, non-disease linked genes or chromosome locations and the size differences are between 4-6 bp to enable amplifying several such InDels in one single multiplex PCR with rather short fragments. These fragments are distinguished from each other using fluorescence detection after capillary electrophoretic separation.
GT InDel Detector markers are designed in such a way that the distribution of loci covers most of the autosomal chromosomes. Markers heterozygosity and SNP in their primer sites were tested on several hundred DNA samples to reduce possible allele dropouts during PCR.
GT InDel Detector Kit comprises primers in a multiplex format which amplifies 30 bi-allelic insertion/deletion markers and AMXY as a sex-determination marker.
The kit is used for human profiling in complex forensic cases and paternity cases. The kit is also used on aged, old, and degraded DNA since most markers produce small fragments.
This kit is compatible with ABI 3500/xL and ABI 3130 platforms for detection and analysis.
- Easy to use mix
- Multiplex analysis of 31 loci in one reaction ;
- Highly specific primers produce sharp peaks with no non-specific peaks;
- Applicable to a variety of DNA sources – blood, hair, bone, saliva, etc., or direct sample to PCR using DNA banking card ( or DBC Blue®, as well as direct amplification of Blood, Amniotic Fluid, and other cultured cells, Chorionic Villi and other tissues suing these lysis buffers (i.e. GT BLB, GT AFLB and GT CVLB);
The table below shows markers in the GT InDel Detector Kit. Names, dyes used, size range, and chromosome locations are shown. The kit is ideal for forensic and human identification in special cases.
This kit is compatible with Applied Biosystems™ 3500/xL, 3130/xl, and SeqStudio platforms for detection and analysis. GT InDel Detector performance has been tested on more than 400 samples.
The kit can be used on DNA extracted from various sources as well as DNA obtained using either hair roots, bone DNA, or direct sample to PCR (extraction free) like GT BLB (Blood Lysis Buffer), and filter papers (GT DBC or GT DBC Blue).
The kit contains all the necessary reagents and buffers for multiplex PCR (Box A) including Primer mix, PCR Master mix, and GT HS Taq DNA Pol. Also, the kit comes with GT InDel Detector Allelic Ladder, GTM5 Matrix Standard for calibrating the Genetic Analyzer as well as GT500 size standard (Box B).