AneuSure® Plus

SKU: GT-11102 Categories: ,

AneuSure® Plus Kit is a QF-PCR kit consisting of 26 markers for rapid prenatal diagnosis of chromosomal aneuploidy and 2 markers for 5q SMA detection.

AneuSure® Plus Kit is a QF-PCR kit consisting of 26 markers for rapid prenatal diagnosis of chromosomal aneuploidy and 2 markers for 5q SMA detection. The aneuploidy markers are for chromosomes 21 (Down Syndrome or +21), 18 (Edwards Syndrome or +18), 13 (Patau Syndrome or +13) as well as 47, XXY (Klinefelter Syndrome), 47, XXX (Triple X Syndrome), 45, X (Turner Syndrome) and other sex chromosomes. The AneuSure® Plus QF-PCR STR markers are distributed across autosomal chromosomes 21, 18 and 13 and sex chromosomes X & Y. The segmental duplication 7X marker is included in the kit for the differentiation of X chromosome monosomy from homozygosity (i.e., it quantifies chromosome X for more accurate detection of Turner Syndrome).

AneuSure® Plus kit also includes markers for the detection of 5q deletion or conversion on exon 7 of SMN1 and SMN2. Spinal Muscular Atrophy (SMA) is one of the most prevalent neuromuscular disorders. The disease is inherited as an autosomal recessive disorder.

Premium Features

  • Simultaneous detection of common chromosomal aneuploidies and deletion or conversion of exon 7 of SMN1 gene cause of SMA;
  • Multiplex analysis of 28 markers in one reaction;
  • Rapid diagnostic follow-up after NIPT tests;
  • Accurate detection of Turner Syndrome;
  • Applicable to a variety of DNA sources – amniotic fluid (AF), chorionic villus (CVS), fetal tissue, etc.;
  • Can be used by any of Genetek Direct PCR products like GT DBC, GT DBC Blue, as well as direct amplification of Blood, Amniotic Fluid and other cultured cells, Chorionic Villi and other tissues suing these lysis buffers (i.e., GT BLB, GT AFLB and GT CVLB);
  • Easy to use mix

For research use only. Not for use in diagnostic procedures.

Markers

D21S1809, D21S1446, D21IFNAR, D21S1414, D21S1442, D21S1411, D18S390, D18S391, D18S1002, D18S535, D18-GATA178F11, D13S325, D13S252, D13S634, D13S258, D13S797, DXS7132, HPRT, DXS6803, 7X, DX-TATC 13.3, DXS981, AMXY, SRY, Y/X b, DYS437, SMN1 and SMN2 totaling 28 markers.

Panel showing markers and the dye used in AneuSure® Plus QF-PCR kit. The picture also shows GT500 size standard. The kit is a 5-dye system to be used with CE Genetic Analyzers. This QF-PCR kit is used to detect the Down Syndrome or trisomy 21, trisomy 18 or Edwards Syndrome, trisomy 13 or Patau Syndrome, X and Y chromosome aneuploidy like Klinefelter Syndrome, XXY, XXX, Turner Syndromes, as well as SMA. It is also intended for rapid diagnostic follow-up after NIPT test.

Chromosome Locations

Table below shows markers in the AneuSure® Plus Kit. Names, dye used, size range and chromosome locations are shown.

Sample Profile

Quality Control DNADownload
Down Syndrome (+21)Download
Edward Syndrome (+18)Download
Patau Syndrome (+13)Download
Triploidy 69Download
Turner Syndrome (XO)Download
Klinefelter Syndrome (XXY)Download
Triple X Syndrome (XXX)Download
Affected with SMADownload

Comparison Table

The table shows number of markers for each Genetek QF-PCR kit. There are 26 markers in the AneuSure, 28 in the AneuSure Plus, 32 in the AneuSure Extra, 34 in AneuSure Extra Plus, 32 in AneuSure Max and 34 in AneuSure Max Plus kits. There are 7 segmental duplications in the AneuSure Extra and AneuSure Extra Plus. There are 2 segmental duplication and 5 additional STR markers in AneuSure Max and AneuSure® Max Plus kits. The availability of the above-mentioned kits provide medical geneticists with many powerful options to choose from, considering the requirements of each case. Our innovative inclusion of SMA detection power (by addition of SMN1 and SMN2 exon 7 deletion and conversion in AneuSure Plus, AneuSure Extra Plus and AneuSure Max Plus kits) creates a considerable advantage for any laboratory to detect fetuses who may be normal for aneuploidy but affected with a deadly and costly disease like SMA.

FeaturesAneuSure (QF-PCR Kit)AneuSure® Extra (QF-PCR Kit)AneuSure® Max (QF-PCR Kit)AneuSure® Plus (QF-PCR Kit+ SMA)AneuSure® Extra Plus (QF-PCR Kit+ SMA)AneuSure® Max Plus (QF-PCR Kit+ SMA)
Total Markers263232283434
Y markers222222
X markers677677
X/Y markers222222
Chr. 21 markers688688
Chr. 18 markers587587
Chr. 13 markers567567
Segmental Duplication172172
SMA DetectionNoNoNo
5- or 6-dye system566566
Single-tube multiplex
ABI GA Systems3130/xl3500/xLSeqStudio3500/xLSeqStudio3500/xLSeqStudio3130/xl3500/xLSeqStudio3500/xLSeqStudio3500/xLSeqStudio
A table compares Genetek Biopharma’s range of QF-PCR kits for the detection of chromosomal aneuploidies. The fetal samples can be used directly, after first or second trimester tests or follow up after NIPT result. Some kits also detect exon-7 deletion in 5q SMA.

Compatibility

This kit is compatible with 5-dye system of capillary electrophoresis such as with ABI 3130/xl, 3500/xL, SeqStudio Genetic Analyzers with either 30, 50, or 80 capillaries.

This kit can be used on various sources of DNA including DNA extracted from various sources or DNA obtained using any of extraction free (direct) materials from Genetek such as GT AFLB (Amniotic Fluid Lysis Buffer), GT CVLB (Chorionic Villus Lysis Buffer, GT BLB (Blood Lysis Buffer), and filter papers (GT DBC or GT DBC Blue).

Kit Content

The kit contains all the necessary reagents and buffers for multiplex PCR (Multiplex Primer mix, PCR buffer, GT HSTaq DNA Pol in Box A). Also, the kit comes with GT500 size standard as well as GTM5 Matrix Standard for calibrating the Genetic Analyzer (Box B).

User Manuals

AneuSure® Plus Instruction Manual Login to download
GTM5 User Manual Login to download

Quick Protocols

AneuSure® Plus Quick Protocol Login to download
GTM5 Quick Protocol Login to download
GT500 Quick Protocol Login to download

Analysis Assistant

GeneMapper ID v.3.2 Login to download
GeneMapper IDX v.1.3 Login to download
GeneMapper IDX v.1.4 Login to download
GeneMapper IDX v.1.5 Login to download
GeneMapper IDX v.1.6 Login to download
GeneMapper v6 Login to download
GeneMarker Login to download

MSDS (SDS)

PCR Mix Login to download
Primer Mix Login to download
HSTaq Login to download
GT QCDM Login to download
PCR Grade Water Login to download
GT500 Size Standard Login to download
GTM5 Matrix Standard Login to download

Brochure / Catalog

AneuSure® Brochure​ Login to download
AneuSure® Plus Brochure​ Login to download
AneuSure® Extra Brochure​ Login to download
AneuSure® Extra Plus Brochure Login to download
AneuSure® Max Brochure​ Login to download
AneuSure® Max Plus Brochure​ Login to download

Size SKU. No. Order
50 RXN GT-11102-50 Order Now
100 RXN GT-11102-100 Order Now
1000 RXN GT-11102-1K Order Now
10000 RXN GT-11102-10K Inquire

There are numerous studies on the use of QF-PCR. One detailed “Best Practice Guideline” for using QF-PCR is “ACGS best practice guidelines for use of Quantitative Fluorescence-PCR for the detection of aneuploidy” by Mann et al. (2018).

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