Male Profiling in Sexual Assault, Forensic Casework Samples, Paternity Cases, and Research use such as Ancestry Studies
The GT YDetector 29 multiplex system has been designed to achieve high power of discrimination using a 5-dye system, without upgrading the detection system to six dye channels. This Kit is optimized to amplify 29 STR markers plus AMXY in a single reaction. Additionally, an Amelogenin marker is used to eliminate female sample contamination or PCR failure due to female sample mix-up. The Kit allows the detection of the low amount of male DNA in the female sample (important for sexual assault cases) as well as the detection of male-male DNA mixtures.
- 29 Y chromosome specific markers in a five-dye format allows it to be used on ABI 3130/xl as well as 3500/xL Genetic Analyzers;
- Easy to use mix;
- Multiplex analysis of 30 loci in 5 dye system;
- AMXY loci is added to detect mixed sample source (male-female mix) and to rule out female DNA contamination in sexual assault;
- Applicable to a variety of DNA sources – blood, hair, bone, saliva, etc. or direct sample to PCR using DNA banking card (DBC®) or DBC Blue®.
The table below shows markers in the GT YDetector 29 Kit. Names, dyes used, size range, and chromosome locations are given.
This Kit is compatible with Applied Biosystems™ 3500/xL and 3130/xl capillary electrophoresis platforms for detection and analysis. The kit’s performance has been tested on more than 1000 samples which some have been deposited in the YHRD database.
The kit can be used on DNA extracted from various sources as well as DNA obtained using either hair roots, bone DNA, or direct sample to PCR (extraction free) like GT BLB (Blood Lysis Buffer), and filter papers (GT DBC or GT DBC Blue).
The kit contains all the necessary reagents and buffers for multiplex PCR (Multiplex Primer mix, PCR buffer, GT HSTaq DNA Pol in Box A). Also, the kit comes with GT YDetector 29 Allelic Ladder, GT500 size standard as well as GTM5 Matrix standard for calibrating the Genetic Analyzer (Box B).