GT AZFScreen v2

The GT AZFScreen v2 kit is an STS-based (Sequence-Tagged Sites) kit for rapid and accurate detection of Y chromosome microdeletions in cases of male infertility. GT AZFScreen v2 kit is an updated AZF kit to comply with the EAA/EMQA 2023 Best Practice Guideline. It is intended for the detection of Y chromosome microdeletions, a frequent genetic cause of infertility in men. Infertility is regarded as a critical problem for most couples. It affects 15-20 % of reproductive-age couples worldwide. Male infertility contributes to about 20-30% of these cases, out of which Y chromosome microdeletions cause 5-10 %.

The GT AZFScreen v2 kit includes 29 markers (including 26 STS, 2 SD [Segmental Duplication] and 1 STR. The primer design followed European Molecular Genetics Quality Network guidelines.

The GT AZFScreen v2 kit can also detect Klinefelter Syndrome (47, XXY), another cause of male infertility in men. However, we recommend the use of GT AZFScreen Plus v2 kit for more accurate detection of Klinefelter Syndrome. GT AZFScreen v2 kit performance has been validated with extensive testing using Applied Biosystems™ 3500/xL and 3130/xl platforms for detection and analysis on more than 500 samples, either as normal, with Klinefelter Syndrome, deletions of AZFa, AZFb, and AZFc or any combination of these deletions.

GT AZFScreen v2 Premium Features

• Multiplex analysis of 29 markers in a single reaction which makes it less expensive and less lab work
• For rapid and accurate detection of deletions in any of AZF-related regions on the human Y chromosome as well as markers for Klinefelter Syndrome detection
• Rapid testing for AZF-related infertility in men as well as screening for Klinefelter Syndrome
• Applicable to a variety of DNA sources including extracted DNA or using our DNA extraction-free materials such as blood on filter paper like GT DBC, GT DBC Blue, and lysis buffer like GT BLB
• Easy to use mix and rapid PCR to result
• Easy to interpret results
• For research use only. Not for use in diagnostic procedures.

Markers

sY153 (AZFb), sY1191 (AZFb), sY127 (AZFb), sY157 (AZFc), sY625 (AZFa), sY1224 (AZFb), sY105 (AZFb), sY1192 (AZFb), Y/X b, sY90 (internal control), sY121 (AZFb), sY160 (Heterochromatin), sY1064 (AZFa), M259 (AZFa), SRY(SY14), sY130 (AZFb), AMXY, sY86 (AZFa), ZFY/X, sY84 (AZFa), sY82 (AZFa), sY1065 (AZFa), sY254 (AZFc), sY134 (AZFb), sY255 (AZFc), DXS7132, sY131 (AZFb), sY1182 (AZFa), sY88 (AZFa)

Chromosome Locations

Table below shows markers in the GT AZFSCreen Kit. Names, dye used, size range and chromosome locations are shown.

Sample Profile

The table shows downloadable files for the GT AZFScreen v2 kit. The files can be downloaded after registering. Click on the login to download to register.

Comparison Table

The table compares Genetek Biopharma’s GT AZFScreen v2 kits for the detection of deletion in the AZF region of the Y-chromosome as well as the Klinefelter syndrome in male infertility. The kit is based on EAA/EMQN 2023 Best Practice Guidelines for molecular diagnosis of Y-chromosome microdeletion

Compatibility

This kit is compatible with 5-dyes capillary electrophoresis system such as with Compact Spectrum CE System from Promega or ABI 3130/xl, 3500/xL , and SeqStudio Genetic Analyzers with either 30, 50, or 80 capillaries.

This kit can be used on various sources of DNA including DNA extracted from various sources or DNA obtained using any of extraction free (direct) materials from Genetek such as GT BLB (Blood Lysis Buffer), and filter papers (GT DBC or GT DBC Blue).

Kit Content

The kit contains all the necessary reagents and buffers for multiplex PCR (Multiplex Primer mix, PCR buffer, GT HSTaq DNA Pol in Box A). Also, the kit comes with the GT500 internal size standard as well as GTM5 v2 Matrix Standard for Spectral Calibration of CE systems.