GT AZFScreen Plus

SKU: GT-11302 Categories: ,

GT AZFScreen Plus kits has more markers to detect AZF and 47 XXY trisomy or Klinefelter syndrome by having several X-chromosome STR, segmental duplication, sex-typing markers.

GT AZFScreen Plus

GT AZFScreen Plus kit is developed for the detection of Y chromosome microdeletions and Klinefelter Syndrome, the two most frequent genetic causes of infertility in men. This kit includes 28 markers (16 STS markers, 4 SD [Segmental Duplication] and 8 STR [Short Tandem Repeat]). Primer designing followed European Molecular Genetics Quality Network guidelines.

Infertility is regarded as a critical problem for most couples. It affects 15-20 % of

reproductive-aged couples worldwide. Male infertility contributes to about 20-30 % of these cases, out of which Y chromosome microdeletions cause 5-10 %. About 3 % of infertile men and 14 % of non-obstructive azoospermia men are affected by Klinefelter Syndrome.

The GT AZFScreen plus kit having 8 X chromosome specific STR markers as well as two segmental duplications (7/X and 18/X) can detect Klinefelter Syndrome (47, XXY) and other X or Y chromosome aneuploidies (e.g., 47, XXX, 48, XXXY, 48, XXYY, 48, XXXX, etc.). GT AZFScreen Plus kit performance has been validated with extensive testing using Applied Biosystems™ 3500/xL and 3130/xl platforms for detection and analysis on more than 200 samples, either as normal, with Klinefelter Syndrome, deletions of AZFa, AZFb, AZFc, AZFd or any combination of these deletions on Y chromosome as well as other X chromosome aneuploidies. Please consult the GT AZFScreen Plus user manual for more detailed information.

GT AZFScreen Plus Premium Features

  • For rapid and accurate detection of deletions in any of AZF related regions on human Y chromosome as well as markers for accurate Klinefelter Syndrome detection;
  • Multiplex analysis of 28 markers in single reaction which makes it less expensive and less of lab work;
  • Applicable to a variety of DNA sources including our DNA extraction free materials such as blood on filter paper like GT DBC, GT DBC Blue, and GT BLB
  • Easy to use mix and rapid PCR to result;
  • Easy to interpret results.

For research use only. Not for use in diagnostic procedures.

Markers

SY153(AZFd), SY127 (AZFb), 7/X, SY157 (AZFc), SY625(AZFa), DXS10101, DXS10146, X/Y b, SY90, DXS7423, DXF8SU6, M259 (AZFa), SRY(SY14), SY130 (AZFb), AMXY, SY86 (AZFa), ZFX/Y, SY84 (AZFa), 18/X, SY254 (AZFc), SY134 (AZFb), SY255 (AZFc), DXS7132, SY131 (AZFb), SY152(AZFd), DXS10079, DXS981 and DXS8378.

Panel showing markers and the dye used in GT AZFScreen Plus kit. The picture also shows GT500 size standard. The kit is a 5-dye system to be used with CE Genetic Analyzers. This kit detects deletions seen in the AZF regions of the human Y chromosome in male infertility. It also has several segmental and STR markers for the detection of Klinefelter Syndrome of 47, XXY.

Chromosome Locations

Table below shows markers in the GT AZFScreen Plus Kit. Names, dye used, size range and chromosome locations are shown.

GT AZFScreen Plus markers’ distribution on human X Chromosome
GT AZFScreen Plus markers’ distribution on human Y Chromosome

Sample Profile

Quality Control DNADownload
AZFa MicrodeletionsDownload
AZFb MicrodeletionsDownload
Klinefelter Syndrome (XXY)Download
Turner Syndrome (XO)Download

Comparison Table

The table compares Genetek Biopharma’s AZFScreen kits for the detection of deletion in the AZF region of the Y-chromosome in male infertility as well as detection of Klinefelter syndrome.

Compatibility

This kit is compatible with 5-dyes capillary electrophoresis system such as with ABI 3130/xl, 3500/xL Genetic Analyzers with either 30, 50, or 80 capillaries.

This kit can be used on various sources of DNA including DNA extracted from various sources or DNA obtained using any of extraction free (direct) materials from Genetek such as GT AFLB (Amniotic Fluid Lysis Buffer), GT CVLB (Chorionic Villus Lysis Buffer, GT BLB (Blood Lysis Buffer), and filter papers (GT DBC or GT DBC Blue).

Kit Content

The kit contains all the necessary reagents and buffers for multiplex PCR (Multiplex Primer mix, PCR buffer, GT HSTaq DNA Pol in Box A). Also, the kit comes with GT500 size standard as well as GTM5 Matrix Standard for calibrating the Genetic Analyzer (Box B).

User Manuals

GT AZFScreen Plus User Manual Login to download
GTM5 User Manual Login to download

Quick Protocols

GT AZFScreen Plus Quick Protocol Login to download
GTM5 Quick Protocol Login to download
GT500 Quick Protocol Login to download

Analysis Assistant

GeneMapper ID v.3.2 Login to download
GeneMapper IDX v.1.3 Login to download
GeneMapper IDX v.1.4 Login to download
GeneMapper IDX v.1.5 Login to download
GeneMapper IDX v.1.6 Login to download
GeneMapper v6 Login to download
GeneMarker Login to download

MSDS (SDS)

PCR Mix Login to download
Primer Mix Login to download
HSTaq Login to download
GT QCDM Login to download
PCR Grade Water Login to download
GT500 Size Standard Login to download
GTM5 Matrix Standard Login to download

Brochure / Catalog

GT AZFScreen ‌Brochure Login to download
GT AZFScreen Plus Brochure Login to download

Size SKU. No. Order
50 RXN GT-11302-50 Order Now
100 RXN GT-11302-100 Order Now
1000 RXN GT-11302-1K Order Now
10000 RXN GT-11302-10K Inquire

For further information on mal infertility , tests and AZF please refer to: European Association of Urology (EAU) Guideline on Male Infertility (2019) by A. Jungwirth et. al., and EAA/EMQN best practice guidelines for molecular diagnosis of Y-chromosomal microdeletions: state-of-the-art 2013 by C Krausz  et. al., European Molecular Genetics Quality Network.

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