GT AZFScreen Plus
GT AZFScreen Plus kit is developed for the detection of Y chromosome microdeletions and Klinefelter Syndrome, the two most frequent genetic causes of infertility in men. This kit includes 28 markers (16 STS markers, 4 SD [Segmental Duplication] and 8 STR [Short Tandem Repeat]). Primer designing followed European Molecular Genetics Quality Network guidelines.
Infertility is regarded as a critical problem for most couples. It affects 15-20 % of
reproductive-aged couples worldwide. Male infertility contributes to about 20-30 % of these cases, out of which Y chromosome microdeletions cause 5-10 %. About 3 % of infertile men and 14 % of non-obstructive azoospermia men are affected by Klinefelter Syndrome.
The GT AZFScreen plus kit having 8 X chromosome specific STR markers as well as two segmental duplications (7/X and 18/X) can detect Klinefelter Syndrome (47, XXY) and other X or Y chromosome aneuploidies (e.g., 47, XXX, 48, XXXY, 48, XXYY, 48, XXXX, etc.). GT AZFScreen Plus kit performance has been validated with extensive testing using Applied Biosystems™ 3500/xL and 3130/xl platforms for detection and analysis on more than 200 samples, either as normal, with Klinefelter Syndrome, deletions of AZFa, AZFb, AZFc, AZFd or any combination of these deletions on Y chromosome as well as other X chromosome aneuploidies. Please consult the GT AZFScreen Plus user manual for more detailed information.
GT AZFScreen Plus Premium Features
- For rapid and accurate detection of deletions in any of AZF related regions on human Y chromosome as well as markers for accurate Klinefelter Syndrome detection;
- Multiplex analysis of 28 markers in single reaction which makes it less expensive and less of lab work;
- Applicable to a variety of DNA sources including our DNA extraction free materials such as blood on filter paper like GT DBC, GT DBC Blue, and GT BLB
- Easy to use mix and rapid PCR to result;
- Easy to interpret results.
For research use only. Not for use in diagnostic procedures.
Markers
SY153(AZFd), SY127 (AZFb), 7/X, SY157 (AZFc), SY625(AZFa), DXS10101, DXS10146, X/Y b, SY90, DXS7423, DXF8SU6, M259 (AZFa), SRY(SY14), SY130 (AZFb), AMXY, SY86 (AZFa), ZFX/Y, SY84 (AZFa), 18/X, SY254 (AZFc), SY134 (AZFb), SY255 (AZFc), DXS7132, SY131 (AZFb), SY152(AZFd), DXS10079, DXS981 and DXS8378.
Chromosome Locations
Table below shows markers in the GT AZFScreen Plus Kit. Names, dye used, size range and chromosome locations are shown.
Sample Profile
| Quality Control DNA | Download |
| AZFa Microdeletions | Download |
| AZFb Microdeletions | Download |
| Klinefelter Syndrome (XXY) | Download |
| Turner Syndrome (XO) | Download |
Comparison Table
The table compares Genetek Biopharma’s AZFScreen kits for the detection of deletion in the AZF region of the Y-chromosome in male infertility as well as detection of Klinefelter syndrome.
Compatibility
This kit is compatible with 5-dyes capillary electrophoresis system such as with ABI 3130/xl, 3500/xL Genetic Analyzers with either 30, 50, or 80 capillaries.
This kit can be used on various sources of DNA including DNA extracted from various sources or DNA obtained using any of extraction free (direct) materials from Genetek such as GT AFLB (Amniotic Fluid Lysis Buffer), GT CVLB (Chorionic Villus Lysis Buffer, GT BLB (Blood Lysis Buffer), and filter papers (GT DBC or GT DBC Blue).
Kit Content
The kit contains all the necessary reagents and buffers for multiplex PCR (Multiplex Primer mix, PCR buffer, GT HSTaq DNA Pol in Box A). Also, the kit comes with GT500 size standard as well as GTM5 Matrix Standard for calibrating the Genetic Analyzer (Box B).
