GT Chimerism Detector

The GT Chimerism Detector kit has been developed to meet the strict requirements for the detection of chimerism (engraftment analysis) after bone marrow transplantation (BMT). BMT is done for more than 40 different diseases, including leukemias, thalassemia, Sickle Cell disease, etc. In BMT, usually, the genetic makeup of donor is different from the recipient. This is particularly more important when BMT has been carried out for blood cancers (i.e., leukemia, lymphoma, and myeloma). In these cases, physicians need to ascertain that the recipient cells have been replaced by the donor’s cells or there is mixture of both cell types. The best method is to look at molecular differences (e.g., STRs, short tandem repeats and SNPs, single nucleotide polymorphisms).

Genetek has developed GT Chimerism Detector, a fluorescently based multiplex PCR, a kit which has 16 STR markers (CSF1PO, FGA, TH01, TPOX, vWA, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D21S11, D19S433, D2S1338 and SE33) and AMXY (sex markers). This kit allows the user to have a distinct profile for each person. The profile is like those used in forensic genetics and human identification. If two or more DNA samples are mixed with one another, then one will see more than two peaks for each marker or locus. In chimerism tests, after BMT, physicians are looking to see if there is one set of DNA profile or if there are two sets, one like the donor’s and the other like the recipients, though with different heights of peaks.

GT Chimerism Detector Premium Features

• Easy to use mix;
• Multiplex analysis of 17 loci in one reaction;
• High discriminatory power of each marker;
• Easy to distinguish each persons’ profile by having allelic ladder and bin sets;
• Since designed in a 5-dye system, it is compatible with a broad range of capillary electrophoresis systems, namely 3130/xl, 3500/xL and Seq Studio.

For research use only. Not for use in diagnostic procedures.

Product Details

The GT Chimerism Detector kit has been developed to meet the strict requirements for the detection of chimerism (engraftment analysis) after bone marrow transplantation (BMT). BMT is done for more than 40 different diseases, including leukemias, thalassemia, Sickle Cell disease, etc. In BMT, usually, the genetic makeup of donor is different from the recipient. This is particularly more important when BMT has been carried out for blood cancers (i.e., leukemia, lymphoma, and myeloma). In these cases, physicians need to ascertain that the recipient cells have been replaced by the donor’s cells or there is mixture of both cell types. The best method is to look at molecular differences (e.g., STRs, short tandem repeats and SNPs, single nucleotide polymorphisms).

Genetek has developed GT Chimerism Detector, a fluorescently based multiplex PCR, a kit which has 16 STR markers (CSF1PO, FGA, TH01, TPOX, vWA, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D21S11, D19S433, D2S1338 and SE33) and AMXY (sex markers). Using this kit, will allow the user to have a profile for each person. The profile is like those used in forensic genetics and human identification. If two or more DNA samples are mixed with one another, then one will see more than two peaks for each marker or locus. In chimerism tests, after BMT, physicians are looking to see if there is one set of DNA profile or if there are two sets, one like the donor’s and the other like the recipients.

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This kit is based on the use of STR markers accepted and recommended by the international organizations such as European Network of Forensic Science Institutes (ENFSI), National Institute of Standard Technology (NIST), International Society of Forensic Genetics (ISFG), etc. Therefore, these markers have high heterozygosity for most populations.

Most of these highly polymorphic markers have been validated on more than 200 HLA-matched related donor-recipient by Thiede et al. Genetek Biopharma’s internal validation tests show that the kit is very sensitive and recipient DNA fractions of more than 5% can be detected. This will allow monitoring the presence of residual cells or recurrence (e.g., in cases of Leukemia).

How is it done?

The test involves identifying the STR profiles of the donor and the recipient both before transplantation takes place and after to evaluate the extent of mixture in the recipient’s blood or bone marrow. To do so, each persons’ profile is determined and after transplantation, the performance of the transplant engraftment is assessed by looking at the STR alleles or profile to evaluate donor versus recipient contribution of the white blood cells in the recipient’s blood or bone marrow.

Markers

There are 17 markers including AMXY.

Chromosome Locations

Compatibility​

This kit is compatible with 5-dye system of capillary electrophoresis such as with ABI 3130/xl, 3500/xL , and Seq Studio Genetic Analyzers with either 30, 50, or 80 capillaries.

This kit can be used on various sources of DNA including DNA extracted from various sources or DNA obtained using any of extraction free (direct) materials from Genetek such as GT AFLB (Amniotic Fluid Lysis Buffer), GT CVLB (Chorionic Villus Lysis Buffer, GT BLB (Blood Lysis Buffer), and filter papers (GT DBC or GT DBC Blue).

Kit Content

The kit contains all the necessary reagents and buffers for multiplex PCR (Multiplex Primer mix, PCR buffer, GT HSTaq DNA Pol in Box A). Also, the kit comes with GT500 size standard, GTM5 Matrix Standard for calibrating the Genetic Analyzer and GT Chimerism Detector Allelic Ladder (Box B).