GT HBA Gap Detector

Alpha-globin gene mutations sometimes can cause anemia such as H-Disease or more severe form causes Hydrops Fetalis which is inherited as an autosomal recessive manner.  Alpha-globin disorders are more prevalent in Southeast Asia but is also common in the Middle East and the Mediterranean regions. The majority of alpha-thal carriers have deletions. Mutations like 3.7kb, 4.2kb, 20.5kb, and MED deletions are more prevalent in the Middle East and the Mediterranean regions. Most cases of beta-thal carriers with normal HBA2 level, have similar MCV, MCH, RBC and HBA levels. Therefore, it is sometimes necessary to rule out alpha-thalassemia from beta-thalassemia during premarital, preconception, and pregnancy screenings. On the other hand, imbalances between the alpha and beta-globin copies can cause disease more often called beta-thal intermedia when one parent has 3.7 triplication and the other is carrier of beta-thal.

The GT HBA Detector is an agarose-based PCR kit to aid detection of common alpha-globin gene deletions (3.7kb, 4.2kb, 20.5kb, and MED). The result interpretation has been facilitated by having an allelic ladder (i.e., has ɑ, 3.7kb, 4.2kb, 20.5kb, and MED fragments) which can be run with the unknown samples.

PCR products are analyzed using 1-1.5% agarose gel electrophoresis. The kit comes with allele specific allelic ladder (i.e., ɑ, 3.7kb, 4.2kb, 20.5kb, and MED fragments).

Storage conditions

•Store all components at -20°C

•Avoid repeated freezing-thawing cycles to maintain the good quality of the kit. We recommend to aliquot the components if necessary.