GT HBA Gap Detector

Alpha-thalassemia is mainly caused by deletions covering Alpha 1 or/and Alpha 2-globin genes. The common mutations in the Middle East and Mediterranean are 3.7 kb, 4.2 kb, 20.5 kb and MED deletions. The spectrum of mutations in the South-East Asia are different. We at Genetek have developed two versions of HBA deletion kits.

Alpha-thalassemia is an autosomal recessive disorder. It is more prevalent in Southeast Asia but is also common in the Middle-East and the Mediterranean regions. Mutations in the alpha-globin genes are responsible for alpha-thalassemia. These mutations (deletions, insertions or point mutations) have similar effects on blood indices like MCV, MCH, etc. therefore, it is sometimes necessary to rule out alpha-thalassemia from beta-thalassemia during premarital, preconception and pregnancy screenings. Rapid detection or ruling in/out alpha-thalassemia from beta-thalassemia can reduce costs or confirm presence of alpha-globin gene defect.

Mutations like 3.7kb, 4.2kb, 20.5kb and MED deletions are more prevalent in the Middle East and the Mediterranean regions. The GT HBA Detector is a PCR-based multiplex kit to detect individuals affected or carrier of common alpha-globin gene deletions (3.7kb, 4.2kb, 20.5kb and MED). GT HBA Gap Detector is a multiplex kit for fast and accurate detection of these deletions whenever alpha-globin mutation is suspected or has to be ruled out/in (e.g., in cases where beta-or alpha-thal must be confirmed).

PCR products are analyzed using 1-1.5% agarose gel electrophoresis. We suggest to use 1 kb ladder for rough sizing of fragments and determination of which deletion(s) is/are present.

Storage conditions

  • Store all components at -20°C
  • Avoid repeated freezing-thawing cycles to maintain the good quality of the kit. We recommend to aliquot the components if necessary.

Quick Protocols

GT HBA Gap Detector Quick Protocol Login to download

MSDS (SDS)

PCR Mix Login to download
Primer Mix Login to download
HSTaq Login to download
GT QCDM102 (αα/αα) Login to download