GT-HapScreen F8 is developed to confirm the detection of Factor VIII deficiency, commonly known as Hemophilia A disorder. The kit functions on the principle based on STR markers and QF PCR technique to detect carrier or affected patient by linkage analysis. Use of STR markers makes prenatal diagnosis more accurate and reliable.
The kit’s components include multiplex PCR primers which are designed from the F8 gene. This gene is commonly used in carrier detection and prenatal diagnosis of Factor VIII deficiency. STR markers are designed from 5 regions that cover the upstream and downstream of the F8 gene.
This kit is optimised to use DNA samples purified from blood, amniotic fluid, and chorionic villus (CVS).
Along with Factor VIII deficiency detection confirmation, the GT-HapScreen F8 kit includes autosomal STR markers for Chromosomes 21, 18, 13, X and Y.
These markers provide extra advantages along with the confirmation of mutation detection, viz:
- Mutant gene determination in multigenic disorders using linkage analysis
- Unambiguous differentiation between affected and unaffected fetuses
- Avoidance of sample cross-contamination
- Ruling out maternal cell contamination
- Chromosomal aneuploidy detection
- Sex determination
- Paternity testing