The GT-HapScreen Dystrophin is developed to confirm the detection of Duchenne or Becker Muscular Dystrophy. The kit functions on the principle based on STR markers and QF PCR technique to detect carrier or affected patient by linkage analysis. The use of STR markers makes prenatal diagnosis more accurate and reliable.
The kit´s components include multiplex PCR primers which are designed from a dystrophin gene. This gene is used commonly in carrier detection and prenatal diagnosis of Duchenne or Becker Muscular Dystrophy. The STR markers are designed from 6 regions that cover the upstream, downstream and intron of the dystrophin gene.
This kit is optimized to use DNA samples purified from blood, amniotic fluid and chorionic villus (CVS).
Along with Duchenne or Becker Muscular Dystrophy detection, the GT-HapScreen Dystrophin kit includes autosomal STR markers for Chromosomes 21, 18, 13, X and Y.
These markers provide extra advantages along with the confirmation of mutation detection, viz:
- Mutant gene determination in multigenic disorders using linkage analysis
- Unambigious differentiation between affected and unaffected fetuses
- Avoidance of sample cross-contamination
- Ruling out maternal cell contamination
- Chromosomal aneuploidy detection
- Sex determination
- Paternity testing