GT-AZFScreen

This kit is developed for detection of Y chromosome microdeletions in infertile men. The kit includes 16 markers (13 STS, 2 segmental duplication and 1 STR markers respectively). We design primers on these markers following the European Molecular Genetics Quality Network guidelines. The 16 loci are amplified in a multiplex QF-PCR to increase sample throughput. The PCR products are analyzed on Capillary electrophoresis using ABI Genetic Analyzer platform.

Why is this test important?

Male infertility is a critical health problem that affects 8 – 12% of reproductive aged couples worldwide. Male infertility contributes to about 20 – 30% of these cases, out of which 5 – 10% are caused by Y chromosome microdeletions.

QUALITY CONTROL PROFILE
AZFa MICRODELETION PROFILE
AZFb MICRODELETION PROFILE
KLINEFELTER PROFILE
AZFScreen_Markers

Science behind the GT-AZFScreen

Microdeletions in the long arm of the Y chromosome can cause spermatogenic failure. The critical region for spermatogenesis is the azoospermia factor (AZF), the deletion of which causes azoospermia. Four loci AZFa, AZFb, AZFc and AZFd required for normal spermatogenesis, are located in the AZF regions. Diagnosing the major cause of azoospermia is an initial and important step in the treatment process for infertile men. Since this kinds of Y chromosome microdeletion can cause different phenotypes, accurate diagnosis is a very important step in the treatment process.

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