For horse identification and profiling
GT-Equine encompasses 17 STR markers, located on 13 chromosomes of equine’s genome. Nine loci are recommended by the International Society of Animal Genetics (ISAG) and eight additional loci are commonly used for horse parentage testing and identification, due to the observed high heterozygosity in different races.
One ready-to-use mix is provided to amplify selected STRs. The 17 loci are amplified in a single multiplex PCR reaction to increase sample throughput. Finally, PCR products can be analyzed with capillary electrophoresis.
DNA sample needed for PCR reaction can be extracted from blood or hair pulled from the mane or tail of horse. Application of an appropriate amount of high quality DNA leads to similar peak sizes. A positive control (equine DNA) and negative control (sterile water) must be included on every PCR run to verify your tests. The positive control is provided for detection of the animal-specific loci.