GT-Taq DNA polymerase is purified from E. coli strain carrying plasmid with the cloned gene encoding Thermus aquaticus DNA polymerase. Taq DNA polymerase catalysis 5’→3’ synthesis of DNA. The enzyme has no detectable 3’→5’ proofreading exonuclease activity but possesses 5’→3’ exonuclease activity.
50 mM Tris-HCl (pH 8.0 at 25°C), 50 mM NaCl, 10 mM MgCl2, 200 μM dATP, 200 μM dCTP, 200 μM dGTP, 50 μM [3H] dTTP, 0,25 mg/ml activated calf thymus DNA.
-20° C in 50 mM Tris-HCl (pH 8.0 at 25°), 50 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% glycerol and 1% triton X-100.
Endo-, exodeoxyribonucleases, ribonucleases free.
• Amplifications of DNA fragments by polymerase chain reaction (PCR).
• DNA labelling with radionucleotides, digoxygenin or biotin.
• DNA sequencing.
One unit of enzyme catalysis incorporation of 10 nanomoles of deoxyribonucleotides into acid-insoluble polynucleotide fraction in 30 min at 70°C.
GT-Taq DNA Polymerase comes in several different sizes. Larger volumes and bulk sales are available, please contact us.